Effective April 1st, 2022, our Saturday Hours will change to 8:00am to 2:00pm. We thank you for your continued support of our practice and we look forward to seeing you soon!

Call us: 865-966–8900. After Hour Emergency: 865-693–4440. Get Directions


Drs. Adele Mays, Kristi Lively, and Laura Sharbel have a special interest in canine reproduction. They have advanced training and extensive experience in assisting breeders with reproductive issues. Their enthusiasm and experience for this aspect of veterinary care is a benefit to patients and pet owners.

In addition to our experience, we utilize proven techniques that have withstood the test of time. 

Canine Breeding & Reproductive Services

Village Veterinary Medical Center currently offers the following canine reproductive services:

  • Comprehensive pre-breeding soundness examination
  • Ovulation timing and troubleshooting
  • LH surge detection
  • Same-day in house progesterone results, Monday through Saturday
  • Vaginal cultures
  • Vaginal cytology
  • Semen collection and morphology evaluation
  • Artificial inseminations: Vaginal AI, transcervical AI, and surgical AI
  • Cesarean deliveries
  • Semen cryopreservation and storage
  • Infertility evaluations
  • Pregnancy ultrasound including fetal heart rates
  • Relaxin Pregnancy testing

Further testing and services for reproduction patients include brucellosis testing, OFA evaluations, leptospirosis testing, thyroid management, eye certifications (CERFs), immunization three-year protocols, and parasite evaluation and prevention. To view AKC podcast series go to:


In addition, Village Veterinary Medical Center is certified by the University of Pennsylvania PennHIP program. When PennHIP evaluations are incorporated into a breeding management program, you can significantly reduce offspring joint laxity and the resulting risk for hip dysplasia in predisposed breeds such as German shepherds and golden retrievers. PennHIP is the gold standard for predicting a dog’s chance of developing osteoarthritis of the hip joints. Learn more about our OFA/PennHIP evaluations.

Artificial Inseminations

Artificial inseminations may be performed by different techniques, fine-tuned to maximize your breeding success depending upon the type of semen being used, the bitch’s timing and age, and the value of the semen being used. Fresh semen can survive for three to seven days in the vaginal vault. Chilled shipped semen may survive approximately three days. Frozen semen only survives for about 6–8 hours once thawed.

Vaginal artificial inseminations

Vaginal artificial inseminations may be performed in the standing bitch once she ovulates. Ovulation takes place typically about 5ng/dl. For fresh side-by-side insemination, or when using chilled shipped semen, vaginal AI may be appropriate. Begin AIs once the bitch ovulates, and then every other day for three to four more breedings, if possible.

Transcervical inseminations

Transcervical inseminations may be performed in the standing bitch, typically without sedation. TCI can be utilized with fresh, chilled shipped, or frozen semen. Best results are seen with fresh or chilled shipped semen. TCI utilizes a scope to pass a catheter through the cervix and deposit the semen into the uterine lumen. The timing of TCI depends upon the type of semen being used. TCI may be combined with other types of insemination to maximize coverage during the fertile period.

Surgical insemination

Surgical insemination is typically only recommended for use with frozen semen, highly valuable semen, or when fertility issues have been a problem. This is an invasive surgical procedure so it has risks not associated with other insemination techniques. If frozen semen is being used, a surgical AI is typically performed about 72 hours after ovulation. Ideally, the progesterone levels during this time are between 15–25ng/dl. Surgical insemination may be combined with other breeding techniques, such as TCI before the surgical procedure is done; however, repeat surgical inseminations are not performed during the same cycle.

About Zoetis

Zoetis canine reproduction offers a comprehensive line of canine reproduction products and storage for equine and canine frozen semen. Zoetis is dedicated to the health of animals. Zoetis reproduction services offer safe and secure global shipping of semen collections.

At Zoetis, there is a network of fifty-six and growing, veterinary facilities that have been certified to assist you with your canine breeding needs. Zoetis has professionally trained these canine Freeze Centers that are conveniently located across the US.

To contact Zoetis about your frozen semen shipping needs, go to:

Contact Us

Village Veterinary Medical Center


11301 Kingston Pike Knoxville, TN 37934

Clinic Hours

Mon-Tues: 7:30 AM - 6:00 PM, Wed: 9:00 AM - 6:00 PM, Thurs-Fri: 7:30 AM - 6:00 PM
Sat: 8:00 AM - 2:00 PM, Sun: CLOSED